RESUMO
In vitro activity of the essential oil from Piper diospyrifolium leaves was tested using disk diffusion techniques. The antifungal assay showed significant potencial antifungal activity: the oil was effective against several clinical fungal strains. The majority compounds in the essential oil were identified as sesquiterpenoids by GC-MS and GC-FID techniques.
Assuntos
Antifúngicos , Técnicas In Vitro , Estruturas Vegetais , Piper/crescimento & desenvolvimento , Piper/genética , Piperaceae/genética , Sesquiterpenos/análise , Árvores , Oceano Atlântico , Métodos , Óleos Voláteis , Folhas de Planta , Preparações de Plantas , MétodosRESUMO
In vitro activity of the essential oil from Piper diospyrifolium leaves was tested using disk diffusion techniques. The antifungal assay showed significant potencial antifungal activity: the oil was effective against several clinical fungal strains. The majority compounds in the essential oil were identified as sesquiterpenoids by GC-MS and GC-FID techniques.
RESUMO
The chemical composition of the essential oil obtained from the leaves of Piperovatum Vahl by hydrodistillation was analyzed by GC-MS. The main constituents found were delta-amorphene (16.5 %), cis-muurola-4(14),5-diene (14.29 %) and gamma-muurolene(13.26%). The crude extracts and isolated compounds were screened for their antimicrobial activity. Hydroalcoholic extracts of different parts of Piper ovatum Vahl, essential oil andamides isolated from leaves were tested against Gram-positive and Gram-negative bacteria and Candida species. All extracts and amides were active against Bacillus subtilis andCandida tropicalis, including clinical strains. Essential oil was active against C. tropicalis.These amides showed an inhibitory effect on the adherence of C. tropicalis ATCC 28707 on cover glasses at 10 microg/mL, but did not show morphological alterations at the tested concentrations. Amides were identified as piperovatine and piperlonguminine, and showed MIC values of 15.6 and 31.2 microg/mL to B. subtilis and 3.9 microg/mL to C. tropicalis, and low toxic effects to Vero cells and macrophages.
Assuntos
Anti-Infecciosos/química , Piper/química , Animais , Anti-Infecciosos/farmacologia , Candida/efeitos dos fármacos , Chlorocebus aethiops , Cromatografia Gasosa-Espectrometria de Massas , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Humanos , Macrófagos/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Folhas de Planta/química , Células Vero/efeitos dos fármacosRESUMO
This study tested in vitro activity of the essential oil from flowers of Calendula officinalis using disk-diffusion techniques. The antifungal assay results showed for the first time that the essential oil has good potential antifungal activity: it was effective against all 23 clinical fungi strains tested.
Neste estudo foi avaliada a atividade antifúngica do óleo essencial, obtido das flores de Calendula officinalis utilizando-se técnica de difusão em discos. Os resultados dos ensaios antifúngicos mostraram pela primeira vez, que o óleo essencial tem um grande potencial antifúngico, pois foi efetivo contra todas as 23 amostras clínicas de fungos testados.
Assuntos
Antifúngicos/análise , Calla (Planta) , Flores , Fungos/isolamento & purificação , Técnicas In Vitro , Óleos Voláteis/análise , MétodosRESUMO
This study tested in vitro activity of the essential oil from flowers of Calendula officinalis using disk-diffusion techniques. The antifungal assay results showed for the first time that the essential oil has good potential antifungal activity: it was effective against all 23 clinical fungi strains tested.
